Article
The X-ray structure of NccX from Cupriavidus metallidurans 31A illustrates potential dangers of detergent solubilization when generating and interpreting crystal structures of membrane proteins.
The Journal of biological chemistry - 7 Nov 2014
Ziani Widade, Maillard Antoine P, Petit-Härtlein Isabelle, Garnier Norbert, Crouzy Serge, Girard Eric, Covès Jacques
Abstract excerpt
The x-ray structure of NccX, a type II transmembrane metal sensor, from Cupriavidus metallidurans 31A has been determined at a resolution of 3.12 Å. This was achieved after solubilization by dodecylphosphocholine and purification in the presence of the detergent. NccX crystal structure did not ma...
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