Article
Crystallization and preliminary X-ray analysis of a truncated mutant of yeast nuclear thiol peroxidase, a novel atypical 2-Cys peroxiredoxin.
Acta crystallographica. Section F, Structural biology and crystallization communications - 1 Jul 2005
Choi Jongkeun, Choi Soonwoong, Choi Jungwon, Cha Mee-Kyung, Kim Il-Han, Shin Whanchul
Abstract excerpt
Saccharomyces cerevisiae nTPx is a thioredoxin-dependent thiol peroxidase that is localized in the nucleus. nTPx belongs to the C-type atypical 2-Cys peroxiredoxin family members, which are frequently called BCPs or PrxQs. A double mutant (C107S/C112S) of nTPx overexpressed in Escherichia coli was spontaneously degraded upon freezing and thawing and its truncated form (residues 57-215; MW = 17837 Da) was...
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