Article
High-accuracy de novo assembly and SNP detection of chloroplast genomes using a SMRT circular consensus sequencing strategy.
The New phytologist - 1 Dec 2014
Li Qiushi, Li Ying, Song Jingyuan, Xu Haibin, Xu Jiang, Zhu Yingjie, Li Xiwen, Gao Huanhuan, Dong Linlin, Qian Jun, Sun Chao, Chen Shilin
Abstract excerpt
A circular consensus sequencing (CCS) strategy involving single molecule, real-time (SMRT) DNA sequencing technology was applied to de novo assembly and single nucleotide polymorphism (SNP) detection of chloroplast genomes. Chloroplast DNA was purified from enriched chloroplasts of pooled individuals to construct a shotgun library for each species. The sequencing reactions were performed on a PacBio RS platform....
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