Article
Analysis of variation in chloroplast DNA sequences.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014
Heinze Berthold, Koziel-Monte Agnieszka, Jahn Daniela
Abstract excerpt
This chapter introduces and reviews methods for analyzing variation in chloroplast DNA, mainly by polymerase chain reaction (PCR) and subsequent revelation of polymorphisms. Sources for chloroplast primers are discussed, as well as methods such as Sanger sequencing, PCR followed by restriction fragment length polymorphism (RFLP), gel electrophoresis, fragment analysis on automated DNA sequencers, denaturing...
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