Article
Capturing chloroplast variation for molecular ecology studies: a simple next generation sequencing approach applied to a rainforest tree.
BMC ecology - 14 Mar 2013
McPherson Hannah, van der Merwe Marlien, Delaney Sven K, Edwards Mark A, Henry Robert J, McIntosh Emma, Rymer Paul D, Milner Melita L, Siow Juelian, Rossetto Maurizio
Abstract excerpt
BACKGROUND: With high quantity and quality data production and low cost, next generation sequencing has the potential to provide new opportunities for plant phylogeographic studies on single and multiple species. Here we present an approach for in silicio chloroplast DNA assembly and single nucleotide polymorphism detection from short-read shotgun sequencing. The approach is simple and effective and can be...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.