Article
Efficient production of (R)-2-hydroxy-4-phenylbutyric acid by using a coupled reconstructed D-lactate dehydrogenase and formate dehydrogenase system.
PloS one - 1 Jan 2014
Sheng Binbin, Zheng Zhaojuan, Lv Min, Zhang Haiwei, Qin Tong, Gao Chao, Ma Cuiqing, Xu Ping
Abstract excerpt
BACKGROUND: (R)-2-hydroxy-4-phenylbutyric acid [(R)-HPBA] is a key precursor for the production of angiotensin-converting enzyme inhibitors. However, the product yield and concentration of reported (R)-HPBA synthetic processes remain unsatisfactory. METHODOLOGY/PRINCIPAL FINDINGS: The Y52L/F299Y mutant of NAD-dependent D-lactate dehydrogenase (D-nLDH) in Lactobacillus bulgaricus ATCC 11842 was found to have high...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.