Article
Evaluation of genetic variability in in vitro sodium azide-induced Dendrobium 'Earsakul' mutants.
Genetics and molecular research : GMR - 24 Jul 2014
Wannajindaporn A, Poolsawat O, Chaowiset W, Tantasawat P A
Abstract excerpt
In vitro mutagenesis of Dendrobium 'Earsakul' was carried out by incubating the protocorm-like bodies in 0-5 mM sodium azide for 1 h. Twenty-eight putative mutants were evaluated for genetic variability compared to untreated control plants using inter-simple sequence repeat (ISSR) analysis. Polymorphic fragments were produced by 9 of 12 ISSR primers. A total of 173 amplified ISSR fragments varying in size from...
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