Article
Screening for mutants of Rhizobium japonicum with defects in nitrogen fixing ability.
Zeitschrift fur Naturforschung. Section C, Biosciences - 1 Jan 2000
Hörcher R, Wilcockson J, Werner D
Abstract excerpt
Mutants of Rhizobium japonicum with reduced ex planta nitrogenase activity could be isolated with high frequency by direct screening of ultra-violet mutagenized bacteria growing as spots on the surface of an appropriate agar medium permitting derepression of nitrogenase synthesis. Small glass chambers fitted with a serum cap were pushed into the agar around each spot of growth, forming a small enclosed gas space...
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