Article
Dynamics of mitochondrial RNA-binding protein complex in Trypanosoma brucei and its petite mutant under optimized immobilization conditions.
Eukaryotic cell - 1 Sept 2014
Huang Zhenqiu, Kaltenbrunner Sabine, Šimková Eva, Stanĕk David, Lukeš Julius, Hashimi Hassan
Abstract excerpt
There are a variety of complex metabolic processes ongoing simultaneously in the single, large mitochondrion of Trypanosoma brucei. Understanding the organellar environment and dynamics of mitochondrial proteins requires quantitative measurement in vivo. In this study, we have validated a method for immobilizing both procyclic stage (PS) and bloodstream stage (BS) T. brucei brucei with a high level of cell...
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