Article
Construction of a library of cloned short tandem repeat (STR) alleles as universal templates for allelic ladder preparation.
Forensic science international. Genetics - 1 Sept 2014
Wang Le, Zhao Xing-Chun, Ye Jian, Liu Jin-Jie, Chen Ting, Bai Xue, Zhang Jian, Ou Yuan, Hu Lan, Jiang Bo-Wei, Wang Feng
Abstract excerpt
Short tandem repeat (STR) genotyping methods are widely used for human identity testing applications, including forensic DNA analysis. Samples of DNA containing the length-variant STR alleles are typically separated and genotyped by comparison to an allelic ladder. Here, we describe a newly devised library of cloned STR alleles. The library covers alleles X and Y for the sex-determining locus Amelogenin and 259...
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