Article
Integrated massively parallel sequencing of 15 autosomal STRs and Amelogenin using a simplified library preparation approach.
Electrophoresis - 1 Jun 2018
Xue Jian, Wu Riga, Pan Yajiao, Wang Shunxia, Qu Baowang, Qin Ying, Shi Yuequn, Zhang Chuchu, Li Ran, Zhang Liyan, Zhou Cheng, Sun Hongyu
Abstract excerpt
Massively parallel sequencing (MPS) technologies, also termed as next-generation sequencing (NGS), are becoming increasingly popular in study of short tandem repeats (STR). However, current library preparation methods are usually based on ligation or two-round PCR that requires more steps, making it time-consuming (about 2 days), laborious and expensive. In this study, a 16-plex STR typing system was designed...
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