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Article

Use of <i>tuf</i> Sequences for Genus-Specific PCR Detection and Phylogenetic Analysis of 28 Streptococcal Species

1 Aug 2004

Abstract excerpt

A 761-bp portion of the tuf gene (encoding the elongation factor Tu) from 28 clinically relevant streptococcal species was obtained by sequencing amplicons generated using broad-range PCR primers. These tuf sequences were used to select Streptococcus-specific PCR primers and to perform phylogenetic analysis. The specificity of the PCR assay was verified using 102 different bacterial species, including the 28...

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PMID: 15297518
DOI: 10.1128/jcm.42.8.3686-3695.2004

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