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Article

Development of a novel multiplexed qPCR and Pyrosequencing method for the detection of human pathogenic yersiniae.

International journal of food microbiology - 18 Sept 2017

Thomas M C, Janzen T W, Huscyzynsky G, Mathews A, Amoako K K

Abstract excerpt

The purpose of this study was to develop a novel and robust molecular assay for the detection of human pathogenic yersiniae (i.e. Yersinia enterocolitica, Y. pseudotuberculosis and Y. pestis) in complex food samples. The assay combines multiplexed real-time PCR (qPCR) and Pyrosequencing for detecting and differentiating human pathogenic yersiniae with high confidence through sequence based confirmation. The assay...

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Identifiers

PMID: 28704728
DOI: 10.1016/j.ijfoodmicro.2017.06.019

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