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Detection of pathogenicYersinia enterocoliticausing the multiplex polymerase chain reaction

1 Aug 1996

Abstract excerpt

A multiplex polymerase chain reaction (PCR) was developed to detect the presence of the ail, yst, and virF genes of Yersinia enterocolitica simultaneously, quickly and accurately. The amplified fragment sizes were 356 base-pairs (bp) for the ail gene, 134 bp for the yst gene, and 231 bp for the virF gene. The specificity of the amplified products was confirmed by hybridization with digoxigenin-labelled...

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PMID: 8760951
DOI: 10.1017/s0950268800001138

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