Article
Evolution of enzymatic activities in the enolase superfamily: galactarate dehydratase III from Agrobacterium tumefaciens C58.
Biochemistry - 1 Jul 2014
Groninger-Poe Fiona P, Bouvier Jason T, Vetting Matthew W, Kalyanaraman Chakrapani, Kumar Ritesh, Almo Steven C, Jacobson Matthew P, Gerlt John A
Abstract excerpt
The genome of Agrobacterium tumefaciens C58 encodes 12 members of the enolase superfamily (ENS), eight of which are members of the mandelate racemase (MR) subgroup and, therefore, likely to be acid sugar dehydratases. Using a library of 77 acid sugars for high-throughput screening, one protein (U...
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