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Staphylococcus aureus Hemolysin A Disrupts Cell–Matrix Adhesions in Human Airway Epithelial Cells

11 Jun 2014

Abstract excerpt

Treatment of primary or immortalized human airway epithelial cells (16HBE14o-, S9) or alveolar cancer cells (A549) with recombinant hemolysin A (rHla), a major virulence-associated factor of Staphylococcus aureus, induces alterations in cell shape and formation of paracellular gaps in the cell layer. Semiquantitative Western blotting using extracts of freshly isolated airway tissue (nasal epithelium) or 16HBE14o-...

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PMID: 24918472
DOI: 10.1165/rcmb.2014-0082oc

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