Article
PCR amplification of SNP loci from crude DNA for large-scale genotyping of oomycetes.
Mycologia - 1 Jan 2000
Hu Jian, Lyon Rebecca, Zhou Yuxin, Lamour Kurt
Abstract excerpt
Similar to other eukaryotes, single nucleotide polymorphism (SNP) markers are abundant in many oomycete plant pathogen genomes. High resolution DNA melting analysis (HR-DMA) is a cost-effective method for SNP genotyping, but like many SNP marker technologies, is limited by the amount and quality of template DNA. We describe PCR preamplification of Phytophthora and Peronospora SNP loci from crude DNA extracted...
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