Article
[Extraction of sperm DNA from mixed stain by the modified differential lysis method combined with silicon bead method].
Fa yi xue za zhi - 1 Feb 2014
Han Hai-Jun, Zhang Yu-Hong, Yang Min, Yi Hai, Yang Geng-Ye, Jia Dong-Tao, Lu Da-Ru
Abstract excerpt
OBJECTIVE: To extract sperm DNA from mixed stain by the modified differential lysis method combined with silicon bead method and to evaluate its application value. METHODS: Fifty-two mixed stains containing female STR genotypes detected by differential lysis method were collected. The sperm DNA was extracted by the modified method combined with silicon bead method, then genotyped with the Identifiler Kit, and...
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