Article
Y-chromosomal haplotyping of single sperm cells isolated from semen mixtures - a successful identification of three perpetrators in a multi-suspect sexual assault case.
Croatian medical journal - 1 Oct 2014
Feng Lei, Xu Cheng, Zeng Xianhai, Zhang Huaicai, Yang Fan, Li Wanshui, Tu Zheng, Li Caixia, Hu Lan
Abstract excerpt
AIM: To obtain individual Y-short tandem repeat (STR) profiles in a multi-suspect sexual assault case. Methods. We used laser cut microdissection to capture the single sperm cell in the multi-contributor semen sample, combined with the low volume polymerase chain reaction (LV-PCR) method to genotype the single sperm cell profiles using the Yfiler(®) kit. Consensus DNA profiles were generated from 5 replicate...
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