Article
[Isolating sperm cells by laser capture microdissection technique from mixture sample].
Fa yi xue za zhi - 1 Feb 2011
Liu Fang, Wang Jing, Yu Li-juan, Guo Jian-zhang, Gao Jun-wei, Jiao Zhang-ping, Tang Hui
Abstract excerpt
OBJECTIVE: To assess the application value of laser capture microdissection (LCM) technique for isolating a small number of sperm cells from mixture sample. METHODS: Mixture samples were prepared with sperm cells and vaginal epithelia at different concentrations. Both LCM technique and the differential lysis method were employed to obtain sperm cells from the mixture samples, and DNA was extracted by magnetic...
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