Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

The effect of Rho kinase inhibition on long-term keratinocyte proliferation is rapid and conditional

28 Apr 2014

Abstract excerpt

INTRODUCTION: We previously demonstrated that the lifespan of primary human keratinocytes could be extended indefinitely by culture in the presence of the Rho kinase (ROCK) inhibitor Y-27632. This technique has proven to be very useful in diverse areas of basic and clinical research. METHODS: In this follow-up study we determine whether the continual presence of Y-27632 is required for sustained proliferation. We...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cancer-related Molecular Pathways
Share to Microtubule and mitosis dynamics
Share to Protein Kinase Regulation and GTPase Signaling

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 24774536
DOI: 10.1186/scrt449

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

The effect of Rho kinase inhibition on long-term keratinocyte proliferation is rapid and conditionalPMID 24774536Read ↗

Human keratinocytes are efficiently immortalized by a Rho kinase inhibitorSemantic proximity Read ↗

Rho Kinase Inhibitor Y-27632 Prolongs the Life Span of Adult Human Keratinocytes, Enhances Skin Equivalent Development, and Facilitates Lentiviral TransductionSemantic proximity Read ↗

Combination of low calcium with Y-27632 rock inhibitor increases the proliferative capacity, expansion potential and lifespan of primary human keratinocytes while retaining their capacity to differentiate into stratified epidermis in a 3D skin model.Semantic proximity Read ↗

Short-term Rho-associated kinase inhibitor treatment accelerates primary keratinocyte growth while preserving stem cell characteristicsSemantic proximity Read ↗

Y-27632 acts beyond ROCK inhibition to maintain epidermal stem-like cells in cultureSemantic proximity Read ↗

Radiation Induces Diffusible Feeder Cell Factor(s) That Cooperate with ROCK Inhibitor to Conditionally Reprogram and Immortalize Epithelial CellsSemantic proximity Read ↗

Prolonged treatment with Y-27632 promotes the senescence of primary human dermal fibroblasts by increasing the expression of IGFBP-5 and transforming them into a CAF-like phenotype.Semantic proximity Read ↗

ROCK inhibition modulates the senescence‐associated secretory phenotype (SASP) in oral keratinocytesSemantic proximity Read ↗

Y-27632-mediated long-term expansion of salivary gland epithelial cellsSemantic proximity Read ↗

Rapamycin retards epigenetic ageing of keratinocytes independently of its effects on replicative senescence, proliferation and differentiationSemantic proximity Read ↗

Immortalization of Primary Keratinocytes and Its Application to Skin ResearchSemantic proximity Read ↗

A two-stage, p16(INK4A)- and p53-dependent keratinocyte senescence mechanism that limits replicative potential independent of telomere status.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.