Article
Crystal structures of GCN2 protein kinase C-terminal domains suggest regulatory differences in yeast and mammals.
The Journal of biological chemistry - 23 May 2014
He Hongzhen, Singh Isha, Wek Sheree A, Dey Souvik, Baird Thomas D, Wek Ronald C, Georgiadis Millie M
Abstract excerpt
In response to amino acid starvation, GCN2 phosphorylation of eIF2 leads to repression of general translation and initiation of gene reprogramming that facilitates adaptation to nutrient stress. GCN2 is a multidomain protein with key regulatory domains that directly monitor uncharged tRNAs which accumulate during nutrient limitation, leading to activation of this eIF2 kinase and translational control. A critical...
Topics
- Animals
- Cells, Cultured
- Crystallization
- Crystallography, X-Ray
- Embryo, Mammalian
- Fibroblasts
- Immunoblotting
- Mice
- Mice, Knockout
- Models, Molecular
- Mutation
- Protein Binding
- Protein Biosynthesis
- Protein Multimerization