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Article

Development of an allele-specific PCR for Escherichia coli B2 sub-typing, a rapid and easy to perform substitute of multilocus sequence typing.

Journal of microbiological methods - 1 Jun 2014

Clermont Olivier, Christenson Julia K, Daubié Anne-Sophie, Gordon David M, Denamur Erick

Abstract excerpt

We developed and validated an allele-specific PCR method for detecting the nine main Escherichia coli phylogroup B2 lineages involved in extra-intestinal infections, which could be used as a substitute for multilocus sequence typing in studies for which the greater resolution at the sequence type level is not needed.

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Identifiers

PMID: 24685601
DOI: 10.1016/j.mimet.2014.03.008

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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