Article
Improved immunoblotting methods provide critical insights into phenotypic differences between two murine dysferlinopathy models.
Muscle & nerve - 1 Aug 2014
Mueller Amber L, Desmond Patrick F, Hsia Ru-Ching, Roche Joseph A
Abstract excerpt
INTRODUCTION: We adopted a proteomics-based approach to gain insights into phenotypic differences between A/J and B10.SJL murine dysferlinopathy models. METHODS: We optimized immunoblotting of dysferlin by preparing homogenates of the tibialis anterior (TA) muscle under several different conditions. We compared TA muscles of control, A/J, and B10.SJL mice for levels of dysferlin; dysferlin's partners MG53,...
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