Article
Proteome-wide search for PP2A substrates in fission yeast.
Proteomics - 1 Jun 2014
Bernal Manuel, Zhurinsky Jacob, Iglesias-Romero Ana B, Sanchez-Romero Maria A, Flor-Parra Ignacio, Tomas-Gallardo Laura, Perez-Pulido Antonio J, Jimenez Juan, Daga Rafael R
Abstract excerpt
PP2A (protein phosphatase 2A) is a major phosphatase in eukaryotic cells that plays an essential role in many processes. PP2A mutations in Schizosaccharomyces pombe result in defects of cell cycle control, cytokinesis and morphogenesis. Which PP2A substrates are responsible for these changes is not known. In this work, we searched for PP2A substrates in S. pombe using two approaches, 2D-DIGE analysis of PP2A...
Topics
- Mutation
- Osmotic Pressure
- Protein Phosphatase 2
- Proteome
- Schizosaccharomyces
- Schizosaccharomyces pombe Proteins
- Substrate Specificity
- Two-Dimensional Difference Gel Electrophoresis
