Article
[Genetically engineered mutants of the envelope protein of the RNA-containing bacteriophage].
Molekuliarnaia biologiia - 1 Jan 2000
Kozlovskaia T M, Pushko P M, Stankevich E I, Dreĭmane A Ia, Sniker D Ia
Abstract excerpt
Expression of the coat protein gene of RNA bacteriophage fr in Escherichia coli cells leads to the formation of capsid-like structures of ca. 25 nm in diameter, which are immunologically indistinguishable from the native phage fr capsids. The modification strategy of the coat protein gene by gene engineering technique was developed in order to localize coat protein regions, which are exposed on the capsid surface...
Topics
- Base Sequence
- Epitopes
- Escherichia coli
- Genes, Viral
- Genetic Engineering
- Immunodiffusion
- Molecular Sequence Data
- Mutation
- Plasmids
- RNA Phages
- Recombinant Proteins