Article
Localization and rearrangement modulation of the N-terminal arm of the membrane-bound major coat protein of bacteriophage M13.
Biochimica et biophysica acta - 20 Dec 2000
Spruijt R B, Meijer A B, Wolfs C J, Hemminga M A
Abstract excerpt
During infection the major coat protein of the filamentous bacteriophage M13 is in the cytoplasmic membrane of the host Escherichia coli. This study focuses on the configurational properties of the N-terminal part of the coat protein in the membrane-bound state. For this purpose X-Cys substitutions are generated at coat protein positions 3, 7, 9, 10, 11, 12, 13, 14, 15, 17, 19, 21, 22, 23 and 24, covering the...
Topics
- Acrylamide
- Amino Acid Sequence
- Amino Acids
- Bacteriophage M13
- Capsid
- Cloning, Molecular
- Cyclic N-Oxides
- Escherichia coli
- Fluorescent Dyes
- Gene Expression
- Genes, Viral
- Lipid Bilayers
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Naphthalenesulfonates
- Phospholipids
- Plasmids