Article
Improved PCR-RFLP method for the identification of Escherichia coli enterohemolysin (ehxA) subtypes.
Journal of microbiological methods - 1 May 2014
Lorenz Sandra C, Fischer Markus, Kase Julie A
Abstract excerpt
Previously published PCR-RFLP methods failed to detect the six genetically different Escherichia coli ehxA subtypes reliably. Here we describe an improved PCR-RFLP method that detects all ehxA subtypes. Importantly, ehxA-subtyping may contribute to the detection of potentially pathogenic STECs and can expedite initial screenings of patient samples and food enrichments.
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