A new pyrosequencing assay for rapid detection and genotyping of Shiga toxin, intimin and O157-specific rfbE genes of Escherichia coli.

doi:10.1016/j.mimet.2014.12.003

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Article

A new pyrosequencing assay for rapid detection and genotyping of Shiga toxin, intimin and O157-specific rfbE genes of Escherichia coli.

Journal of microbiological methods - 1 Feb 2015

Goji Noriko, Mathews Amit, Huszczynski George, Laing Chad R, Gannon Victor P J, Graham Morag R, Amoako Kingsley K

Abstract excerpt

Shiga toxin (stx)-producing Escherichia coli (STEC) contamination in food and water is one of the most recognized concerns and a major financial burden in human hygiene control worldwide. Rapid and highly reliable methods of detecting and identifying STEC causing gastroenteric illnesses are crucial to prevent foodborne outbreaks. A number of tests have been developed and commercialized to detect STEC using...

Topics

Adhesins, Bacterial
Bacteriological Techniques
Canada
Carbohydrate Epimerases
Escherichia coli
Escherichia coli Infections
Escherichia coli Proteins
Food Microbiology
Foodborne Diseases
Genotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genotyping Techniques

Humans

Molecular Typing

Sequence Analysis, DNA

Shiga Toxin

Time Factors

Transaminases

Source

Open in PubMed

Identifiers

PMID: 25523243
DOI: 10.1016/j.mimet.2014.12.003

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-25523243-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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