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Automated 16S Sequencing Using an R-Based Analysis Module for Bacterial Identification

11 Apr 2022

Abstract excerpt

Sanger sequencing of the 16S rRNA gene is widely used as a diagnostic tool for bacterial identification, especially in cases where routine diagnostic methods fail to provide an identification, for organisms that are difficult to culture, or from specimens where cultures remain negative. Our simplified protocol is tailored toward use in frontline laboratories with little to no experience with sequencing. It...

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PMID: 35404089
DOI: 10.1128/spectrum.00408-22

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