Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Construction of an infectious cDNA clone of a swine genotype 3 HEV strain isolated in Shanghai, China.

Intervirology - 1 Jan 2014

Si Fusheng, Shi Biao, Wang Xi, Zhu Yumin, Liu Xia, Yang Qian, Li Zhen

Abstract excerpt

OBJECTIVES: Infectious cDNA clones are important tools for studying molecular mechanisms in RNA viruses. The aim of this study was to construct an infectious cDNA clone for SAAS-JDY5, which is a genotype 3 HEV strain of swine origin. METHODS: Construction employed overlapping PCR and restriction analysis to ligate nine cDNA fragments into a full-length cDNA clone containing 14 mutations compared to the consensus...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to DNA, Complementary
Share to DNA, Viral
Share to Hepatitis E

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Hepatitis E virus

Share to Rats, Sprague-Dawley

Share to Reverse Genetics

Share to Swine Diseases

Source

Identifiers

PMID: 24480875
DOI: 10.1159/000357192

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Construction of an infectious cDNA clone of a swine genotype 3 HEV strain isolated in Shanghai, China.PMID 24480875Read ↗

Full genomic sequence analysis of swine genotype 3 hepatitis E virus isolated from Shanghai.Semantic proximity Read ↗

Capped RNA Transcripts of Full-Length cDNA Clones of Swine Hepatitis E Virus Are Replication Competent When Transfected into Huh7 Cells and Infectious When Intrahepatically Inoculated into PigsSemantic proximity Read ↗

Full genome analysis of swine genotype 3 hepatitis E virus isolated from eastern China.Semantic proximity Read ↗

Infectivity of a genotype 4 hepatitis E virus cDNA clone by intrahepatic inoculation of laboratory rats.Semantic proximity Read ↗

Characterization of Japanese swine and human hepatitis E virus isolates of genotype IV with 99 % identity over the entire genome.Semantic proximity Read ↗

Full-genome nucleotide sequence and analysis of a Chinese swine hepatitis E virus isolate of genotype 4 identified in the Guangxi Zhuang autonomous region: evidence of zoonotic risk from swine to human in South China.Semantic proximity Read ↗

An analysis of two open reading frames (ORF3 and ORF4) of rat hepatitis E virus genome using its infectious cDNA clones with mutations in ORF3 or ORF4Semantic proximity Read ↗

Research on a rat model of genotype IV swine hepatitis E virus.Semantic proximity Read ↗

Analysis of the complete genome of indigenous swine hepatitis E virus isolated in Japan.Semantic proximity Read ↗

The Full-Genome Analysis and Generation of an Infectious cDNA Clone of a Genotype 6 Hepatitis E Virus Variant Obtained from a Japanese Wild Boar: In Vitro Cultivation in Human Cell Lines.Semantic proximity Read ↗

Strategy for efficient generation of numerous full-length cDNA clones of classical swine fever virus for haplotyping.Semantic proximity Read ↗

Determination of the full-genome sequence of hepatitis E virus (HEV) SAAS-FX17 and use as a reference to identify putative HEV genotype 4 virulence determinants.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.