Article
A high-resolution melting analysis for genotyping urogenital Chlamydia trachomatis.
Diagnostic microbiology and infectious disease - 1 Dec 2010
Li Jian-Hong, Yin Yue-Ping, Zheng He-Ping, Zhong Ming-Ying, Peng Rui-Rui, Wang Baoxi, Chen Xiang-Sheng
Abstract excerpt
We have developed a high-resolution melting analysis (HRMA) for the genotyping of Chlamydia trachomatis and applied it specifically to the 11 sexually transmitted infection-related genotypes: D through K and L1 through L3. The variable segment 2 (VS2) was selected as the target for HRMA genotype identification. Eleven C. trachomatis genotypes were amplified by a nested real-time polymerase chain reaction (PCR) in...
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