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Article

Genome analyses of single human oocytes.

Cell - 19 Dec 2013

Hou Yu, Fan Wei, Yan Liying, Li Rong, Lian Ying, Huang Jin, Li Jinsen, Xu Liya, Tang Fuchou, Xie X Sunney, Qiao Jie

Abstract excerpt

Single-cell genome analyses of human oocytes are important for meiosis research and preimplantation genomic screening. However, the nonuniformity of single-cell whole-genome amplification hindered its use. Here, we demonstrate genome analyses of single human oocytes using multiple annealing and looping-based amplification cycle (MALBAC)-based sequencing technology. By sequencing the triads of the first and second...

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PMID: 24360273
DOI: 10.1016/j.cell.2013.11.040

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