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Identification and quantification of benzimidazole resistance polymorphisms in Haemonchus contortus isolated in Northeastern Brazil.

Veterinary parasitology - 31 Jan 2014

dos Santos Jessica Maria Leite, Monteiro Jomar Patrício, Ribeiro Wesley Lyeverton Correia, Macedo Iara Tersia Freitas, Camurça-Vasconcelos Ana Lourdes Fernandes, Vieira Luiz da Silva, Bevilaqua Claudia Maria Leal

Abstract excerpt

Haemonchus contortus is the most prevalent nematode in Brazil. The objective of this study was to select 6 populations of H. contortus of known or suspected benzimidazole resistance status and characterize these using quantitative real-time polymerase chain reaction (qPCR) for single nucleotide polymorphisms (SNPs) F200Y, F167Y and E198A in the β-tubulin isotype 1 gene. qPCR was performed using DNA from a pool of...

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PMID: 24295955
DOI: 10.1016/j.vetpar.2013.11.006

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