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Development and validation of four one-step real-time RT-LAMP assays for specific detection of each dengue virus serotype

29 May 2018

Abstract excerpt

BACKGROUND: 4 one-step, real-time, reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays were developed for the detection of dengue virus (DENV) serotypes by considering 2,056 full genome DENV sequences. DENV1 and DENV2 RT-LAMP assays were validated with 31 blood and 11 serum samples from Tanzania, Senegal, Sudan and Mauritania. DENV3 and DENV4 RT-LAMP assays were validated with 25 serum...

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PMID: 29813062
DOI: 10.1371/journal.pntd.0006381

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