Article
Isolation of zebrafish gonads for RNA isolation.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014
Huang Hsin-Yi, Ketting René F
Abstract excerpt
Piwi proteins and piRNAs are abundant in the gonads of various animal species. Gonads from different developmental stages provide us information regarding the function of piRNAs and the PIWI pathway during germline development. Here we describe methods for gonad and germ cell preparation from different developmental stages of zebrafish. We also describe how to use these gonads to purify and characterize piRNAs.
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