Growth and differentiation of HaCaT keratinocytes.

doi:10.1007/7651_2013_42

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Article

Growth and differentiation of HaCaT keratinocytes.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014

Wilson Van G

Abstract excerpt

HaCaT cells are a spontaneously immortalized, human keratinocyte line that has been widely used for studies of skin biology and differentiation. Under typical culture conditions HaCaT cells have a partially to fully differentiated phenotype due to the high calcium content of both standard media and fetal bovine serum. This chapter describes low-calcium culture conditions for reverting HaCaT cells to the fully...

Topics

Calcium
Cell Culture Techniques
Cell Differentiation
Cell Line
Cell Proliferation
Humans
Keratinocytes
Phenotype

Source

Open in PubMed

Identifiers

PMID: 24155234
DOI: 10.1007/7651_2013_42

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