Article
Demonstration of lipid A-binding proteins on murine lymphoma cells using R-mutant gram-negative bacteria as a detector.
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica - 1 Jan 1990
Nakashima I, Kubota T, Zhang Y, Yoshida T, Inagi R, Isobe K, Nagase F, Ohta M, Kato N, Yokochi T
Abstract excerpt
Some R-mutant Escherichia coli and Salmonella heavily adhered to murine lymphoma cells of B cell and T cell lineages. This adhesion was primarily mediated by membrane-localized proteins on tumor cells, which bind the polymyxin B-reactive hydrophilic structure of lipis A on bacteria. SDS-PAGE analysis of tumor cell membranes showed that proteins or glycoproteins of MW = around 45Kd, 25-35Kd and around 15Kd...
Topics
- Animals
- Bacterial Adhesion
- Carrier Proteins
- Cell Line
- Cell Membrane
- Enzyme-Linked Immunosorbent Assay
- Escherichia coli
- Gram-Negative Bacteria
- Klebsiella pneumoniae
- Lipid A