Article
Rapid genotyping using pyrene-perylene locked nucleic acid complexes.
Artificial DNA, PNA & XNA - 1 Jan 2000
Kumar T Santhosh, Myznikova Anna, Samokhina Evgeniya, Astakhova Irina Kira
Abstract excerpt
We have developed an assay for single strand DNA and RNA detection which is based on novel pyrene-perylene FRET pairs attached to short LNA/DNA probes. The assay is based on ratiometric emission upon binding of target DNA/RNA by three combinations of fluorescent LNA/DNA reporter strands. Specific geometry of the pyrene fluorophore attached to the 2'-amino group of 2'-amino-LNA in position 4 allows for the first...
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