Article
Isolation and functional expression of pituitary peptidylglycine alpha-amidating enzyme mRNA. A variant lacking the transmembrane domain.
FEBS letters - 3 Sept 1990
Kato I, Yonekura H, Yamamoto H, Okamoto H
Abstract excerpt
We demonstrate that, in rat pituitary, peptidylglycine alpha-amidating enzyme was encoded by at least 5 distinct mRNAs. Southern blot and ribonuclease protection analyses revealed that the mRNAs arose through alternative splicing. A variant lacking the transmembrane domain-coding sequence was a m...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Southern
- Cell Line
- Cell Membrane
- DNA
- Gene Expression Regulation, Enzymologic
- Gene Library
- Genetic Variation
- Kinetics
- Mixed Function Oxygenases
- Molecular Sequence Data
- Multienzyme Complexes
- Pituitary Gland
- Plasmids
- RNA, Messenger
- Rats