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Article

Mutations in the QVVAG region of the cysteine proteinase inhibitor stefin B.

Biological chemistry Hoppe-Seyler - 1 May 1990

Jerala R, Trstenjak-Prebanda M, Kroon-Zitko L, Lenarcic B, Turk V

Abstract excerpt

Variants of human stefin B were constructed by cassette mutagenesis. Val47 as the constituent of highly conserved QVVAG sequence was substituted by hydrophobic amino acids of increasing size - Ala, Ile and Phe. Recombinant proteins were expressed in E. coli and Ki values for papain were determined. Substitutions did not cause a major change in Ki value and we conclude that the interaction with the proteinases is...

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PMID: 2400576

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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