Mutations in the QVVAG region of the cysteine proteinase inhibitor stefin B.

Article

Mutations in the QVVAG region of the cysteine proteinase inhibitor stefin B.

Biological chemistry Hoppe-Seyler - 1 May 1990

Jerala R, Trstenjak-Prebanda M, Kroon-Zitko L, Lenarcic B, Turk V

Abstract excerpt

Variants of human stefin B were constructed by cassette mutagenesis. Val47 as the constituent of highly conserved QVVAG sequence was substituted by hydrophobic amino acids of increasing size - Ala, Ile and Phe. Recombinant proteins were expressed in E. coli and Ki values for papain were determine...

Topics

Base Sequence
Cloning, Molecular
Cystatin B
Cystatins
Cysteine Proteinase Inhibitors
Humans
Molecular Sequence Data
Mutation
Papain
Recombinant Proteins

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