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Evaluation of the effectiveness of semen storage and sperm purification methods for spermatozoa transcript profiling

19 Jul 2013

Abstract excerpt

Different semen storage and sperm purification methods may affect the integrity of isolated spermatozoal RNA. RNA-Seq was applied to determine whether semen storage methods (pelleted vs. liquefied) and somatic cell lysis buffer (SCLB) vs. PureSperm (PS) purification methods affect the quantity and quality of sperm RNA. The results indicate that the method of semen storage does not markedly impact RNA profiling...

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PMID: 23869956
DOI: 10.3109/19396368.2013.817626

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