Article
Effect of apoA-I Mutations in the Capacity of Reconstituted HDL to Promote ABCG1-Mediated Cholesterol Efflux.
PloS one - 1 Jan 2013
Daniil Georgios, Zannis Vassilis I, Chroni Angeliki
Abstract excerpt
ATP binding cassette transporter G1 (ABCG1) mediates the cholesterol transport from cells to high-density lipoprotein (HDL), but the role of apolipoprotein A-I (apoA-I), the main protein constituent of HDL, in this process is not clear. To address this, we measured cholesterol efflux from HEK293 cells or J774 mouse macrophages overexpressing ABCG1 using as acceptors reconstituted HDL (rHDL) containing wild-type...
Topics
- ATP Binding Cassette Transporter, Subfamily G, Member 1
- Animals
- Apolipoprotein A-I
- Cell Membrane
- Cholesterol, HDL
- HEK293 Cells
- Humans
- Ketocholesterols
- Mice
- Mutation
- Protein Domains
- Proteolysis
- Recombinant Proteins
- Structure-Activity Relationship