Article
Fish scales and SNP chips: SNP genotyping and allele frequency estimation in individual and pooled DNA from historical samples of Atlantic salmon (Salmo salar).
BMC genomics - 3 Jul 2013
Johnston Susan E, Lindqvist Meri, Niemelä Eero, Orell Panu, Erkinaro Jaakko, Kent Matthew P, Lien Sigbjørn, Vähä Juha-Pekka, Vasemägi Anti, Primmer Craig R
Abstract excerpt
BACKGROUND: DNA extracted from historical samples is an important resource for understanding genetic consequences of anthropogenic influences and long-term environmental change. However, such samples generally yield DNA of a lower amount and quality, and the extent to which DNA degradation affects SNP genotyping success and allele frequency estimation is not well understood. We conducted high density SNP...
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