Bridging the gap between legacy polymerase chain reaction-based microsatellite data with high-throughput sequencing data for conservation genomics.

Abstract excerpt

Microsatellites are powerful markers for tracking genetic variation in wildlife populations due to their high polymorphism and genome-wide abundance. While polymerase chain reaction (PCR)-based fragment size analysis has been the standard for genotyping microsatellites, high-throughput sequencing offers greater resolution and the opportunity to sync historical datasets with modern analyses. We evaluated how...

Topics

• Animals
• Microsatellite Repeats
• High-Throughput Nucleotide Sequencing
• Ursidae
• Polymerase Chain Reaction
• Conservation of Natural Resources