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Article

Isolation of αL I domain mutants mediating firm cell adhesion using a novel flow-based sorting method.

Protein engineering, design & selection : PEDS - 1 Aug 2013

Pepper Lauren R, Parthasarathy Ranganath, Robbins Gregory P, Dang Nicholas N, Hammer Daniel A, Boder Eric T

Abstract excerpt

The inserted (I) domain of αLβ2 integrin (LFA-1) contains the entire binding site of the molecule. It mediates both rolling and firm adhesion of leukocytes at sites of inflammation depending on the activation state of the integrin. The affinity change of the entire integrin can be mimicked by the I domain alone through mutations that affect the conformation of the molecule. High-affinity mutants of the I domain...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 23788664
DOI: 10.1093/protein/gzt028
PMCID: PMC3711393

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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