Extragenic suppressors of paralyzed flagellar mutations in Chlamydomonas reinhardtii identify loci that alter the inner dynein arms.

Abstract excerpt

We have analyzed extragenic suppressors of paralyzed flagella mutations in Chlamydomonas reinhardtii in an effort to identify new dynein mutations. A temperature-sensitive allele of the PF16 locus was mutagenized and then screened for revertants that could swim at the restrictive temperature (Dutcher et al. 1984. J. Cell Biol. 98:229-236). In backcrosses of one of the revertant strains to wild-type, we recovered...

Topics

• Animals
• Chlamydomonas reinhardtii
• Dyneins
• Flagella
• Image Processing, Computer-Assisted
• Microscopy, Electron
• Movement
• Mutation
• Phenotype
• Suppression, Genetic