Article
Mutascope: sensitive detection of somatic mutations from deep amplicon sequencing.
Bioinformatics (Oxford, England) - 1 Aug 2013
Yost Shawn E, Alakus Hakan, Matsui Hiroko, Schwab Richard B, Jepsen Kristen, Frazer Kelly A, Harismendy Olivier
Abstract excerpt
SUMMARY: We present Mutascope, a sequencing analysis pipeline specifically developed for the identification of somatic variants present at low-allelic fraction from high-throughput sequencing of amplicons from matched tumor-normal specimen. Using datasets reproducing tumor genetic heterogeneity, we demonstrate that Mutascope has a higher sensitivity and generates fewer false-positive calls than tools designed for...
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