Article
Detection and genotyping of restriction fragment associated polymorphisms in polyploid crops with a pseudo-reference sequence: a case study in allotetraploid Brassica napus.
BMC genomics - 24 May 2013
Chen Xun, Li Xuemin, Zhang Bing, Xu Jinsong, Wu Zhikun, Wang Bo, Li Haitao, Younas Muhammad, Huang Lei, Luo Yingfeng, Wu Jiangsheng, Hu Songnian, Liu Kede
Abstract excerpt
BACKGROUND: The presence of homoeologous sequences and absence of a reference genome sequence make discovery and genotyping of single nucleotide polymorphisms (SNPs) more challenging in polyploid crops. RESULTS: To address this challenge, we constructed reduced representation libraries (RRLs) for two Brassica napus inbred lines and their 91 doubled haploid (DH) progenies using a modified ddRADseq technique. A...
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