Article
In vitro organ culture of embryonic bursa of Fabricius.
Developmental and comparative immunology - 1 Jan 1990
Nowak J S, Vainio O, Lassila O
Abstract excerpt
An in vitro method for organ culture of embryonic bursa of Fabricius is presented. It is shown that bursa cells proliferate in in vitro culture as evidenced by [3H]-thymidine incorporation. We assessed the expression of B-cell alloantigen (Bu-la and Bu-lb), class I (B-F) and class II (B-L) antigens of chicken major histocompatibility complex (MHC), and surface immunoglobulin (sIg) on cultured bursa cells using...
Topics
- Animals
- Antibodies, Monoclonal
- Antigens, Surface
- B-Lymphocytes
- Bursa of Fabricius
- Cell Division
- Chick Embryo
- Chickens
- Cyclophosphamide
- Lymphocyte Depletion
- Organ Culture Techniques